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Antifungal Effect of Lactic Acid Bacteria
L. Vanne, T. Kleemola, A. Haikara
VTT Biotechnology
[EXTRACT:]
AIM
In this study, the possible antifungal effects of lactic acid bacteria against toxigenic Penicillium and
Aspergillus strains were screened in vitro. The work is a part of the EU project "Prevention of ochratoxin
A in cereals". (QLK1.-CT-1999-00433).
MATERIALS AND METHODS
The mould strains selected for this study were 15 identified Penicillium verrucosum and four Asperillus
ochraceus isolated from wheat and barley. The inhibition of mould growth was screened with seven lactic
acid bacteria (LAB), partly based on earlier studies of their anti fungal effects
A summary of LAB used in this study is presented below:
Nature of the strains
Lactobacillus plantarum
Lactobacillus plantarum
Pediococcus pentosaceus
Pediococcus pentiosaceus
Lactobacillus amylovorus
Lactobacillus amylovorus
Lactobacillus acidophilus
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Strain code / (abbrev.)
VTT E-78076 (E76)
VTT E-79098 (E98)
VTT E-90390 (E390)
VTT E-981081 (E1081)
VTT E-95576 (E576)
VTT E-981145 (E1145)
VTT E-96276 (E276)
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Origin
Beer
Pickled Cabbage
Barley kernel
Oat
Silage
Starch slurry
Human isolate
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The preliminary screening of the anti fungal effect of the LAB was carried out by
automated turbidometry.
RESULTS
The anti funal effect measured by automated turbidometry varied between 20 and 55%.
The set inhibition level of 40% was achieved in 22 of 104 combinations of LAB and
P. verrucosum. The three most effective LAB strains were
Lactobacillus plantarum E76, L. plantarum E98 and L. amylovorus E576.
The inhibition of A. ochraceus by LAB was found to be more than 40% in 11 out of 26
combinations. The inhibition caused by LAB is a combined effect
of lactic acid and anti-microbial substances produced by the bacteria.
All the four LAB strains studied using the indirect impedimetric method were able to
delay or even inhibit the growth of A. ochraceus and P. verruosum during the 90-hour
monitoring period. The most effective strains L. plantarum E76
and L. amylovorus E576, were able to inhibit the growth of all 14 fungal strains tested.
CONCLUSION AND FUTURE PLANS
The results achieved in this study show clearly that the growth of
toxigenic storage fungi can be restricted by LAB in vitro. Further studies
with irradiated barley as growth substrate are going to show the potential of the technique
in barley storage and processing.
REFERENCES
1. Haikara, A and Mattila-Sandholm, T. 1994. Procedure for treatment of seed material
to be germinated. Patent WO9416053 (Pat. FI 94874, 1995)
2. Skytta, E. and Mattila-Sandholm, T. 1991. A quantitative method for assessing
bacteriocins and other food antimicrobials by automated turbidometry.
J. Microbiol. Methods 14, 77-88.
3. Niku-Paavola, M-L., Laitila, A., Mattila-Sandholm, T. and Haikara, A 1999. New types of
antimicrobial compounds produced by Lactobacillus plantarum J. Appl. Microbiol. 86, 29-35.
Comments from healthcare professionals who have used LB17:
Canada
"... I have been using different probiotic blends over the years. The best I found by far is LB17. The ... more
strands of different friendly bateria, the better the probiotic. It has been my experience that LB17 covers every area of body fluids from
the sinuses to the reproductive organs which give LB17 a great advantage over any other probiotic. My patients love the small size of the
capsules and I have used LB17 with great success on open wounds and stubborn acne by ... applying ... directly to the wound.
... be happy to recommend LB17 to any health practitioner."..
Dr. A.W. Martin DC, Ph.D., RNCP, D.N.M. (November 2007)
Dr Martin is a practising nutritionist in Sudbury Ontario with a PhD in nutrition.
United Kingdom
"I am a Holistic Therapy Practitioner in the UK .......
Finally, I want to make it clear that I am extremely pleased with the benefits that this product LB17 provides for myself and my clients and
friends who all wish to purchase more of it..."
Ms Helen Rushforth B.A.(Hons), M.A., Flintshire, North Wales, UK (May 2008)
Ms Helen Rushforth is a practising healthcare professional in North Wales, UK
Below is an image of a box of LB17 live probiotic and vegetable soft gel capsule

Each box contains 60 capsules
LB17 live probiotic biomass
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Above is a 50gm bottle of LB17 biomass. Next pic is image of the biomass in bulk paste form
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The above information is provided for general educational purposes only. It is not intended to replace competent health care advice received
from a knowledgeable healthcare professional. You are urged to seek healthcare advice for the treatment of any illness or disease.
Health Canada and the FDA (USA) have not evaluated these statements. This product is not intended to diagnose, treat, cure, or prevent any
disease.
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